Characterisation of neurons in the pedal ganglia of thegreen-lipped mussel, Perna canaliculus, using antibodiesraised against neuropeptides and neurotransmitters involvedin gastropod egg-laying behaviour and bivalve reproductionand spawning
2008
To characterise central neurons in the pedal ganglia of both male and female green
lipped mussel, Perna canaliculus immunohistochemical techniques were used. Mollusc
antibodies were used against neuropeptides and neurotansmitters known to control
reproduction and spawning. Anti-ELH and anti-APGWamide showed very strong
immunoreactivity in small type of neurons. Anti-5-HT and anti-DA immunoreactivity
was mostly in large type of neurons. The labelled neurons are consistent with
descriptions of neurosecretory cells implicated in the control of reproduction and
spawning on the basis of earlier histological staining techniques used in this species. The
use of selective immunological markers for peptides and amines appears to be a,
promising tool for further characterisation of neurosecretory cells, and to isolate an'tl
characterise neuropeptides and other biologically active materials involved in the control
of reproduction in Perna canaliculus.
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