Compositions, methods and applications related thereto for splitting modified DNA

Composition comprising: one or more purified recombinant proteins, wherein the proteins are members of a family of ZZYZ proteins that are defined by the fact that they have a N-terminal domain comprising SEQ ID NO: 14; and a reaction buffer, wherein the one or more purified proteins with DNA GHMC HMC and / or mC in a ratio of GHMC or HMC mC of at least 8: columns. 1