Rapid identification and recovery of ENU-induced mutations with next-generation sequencing and Paired-End Low-Error analysis
2015
Background
Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics approach to directly identify point mutations in specific genes of interest in genomic DNA from a large chemically mutagenized population. Classical TILLING processes, based on enzymatic detection of mutations in heteroduplex PCR amplicons, are slow and labor intensive.
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