Optimization of incubation condition in the electroporation for the in vitro introduction of hrDNA targeting vector into hepatocyte

Objective:To explore the optimal incubation parameters in the electroporation for the in vitro introduction of hrDNA(human ribosomal DNA) targeting vector into hepatocyte,to improve the transfection efficiency.Methods:Six different incubation conditions were designed,in which all other parameters were identical.The cell survival rates after electroporation and the GFP gene-expressing cell rates 48h after the electroporation were calculated to analyses the effect of different incubation conditions.Result:Among the 6-group incubation conditions,highest transfection efficiency was shown in group with 21℃,1min incubation before electroporation and group with 21℃,1min incubation after electroporation,which were 50% higher than the others.Conclusion:Optimization of incubation condition improved the transfection efficiency of hrDNA targeting vector into hepatocyte.