Construction of infectious clone of porcine circovirus type 2.

To further understand the pathogenesis and gene function of porcine circovirus type 2(PCV2),the full-length genomic sequence of PCV2 was amplified by PCR from the tissues of pigs-infected with PCV2 in Baoding Prefecture,Hebei Province and cloned into pEGFP-N1 vector to generate recombinant plasmid pEGFP-N1-2PCV2 with two copies of the full-length genomic sequence of PCV2.Then,PK-15 cells were transfected with the plasmid by liposome and cultivated by serial 5 blind passages.PCV2 nucleotide could be detected by PCR in the transfected cells,and fluorescent light was observed by the indirect immunofluorescence assay in the third generation cell lines,indicating that PCV2 antigen was expressed in the cells.The results showed that the recombinant plasmid pEGFP-N1-2PCV2 possessed infectivity.