The role of macrophages in increased mitogen response of rat splenic lymphocytes following in vitro incubation with vitamin E.

The role of macrophages (MO) in the enhancement of lymphocyte proliferation by α-tocopherol (VE) was investigated using rat splenocytes. The proliferation of whole splenocytes was significantly higher than that of MO-depleted splenocytes at all concentrations of concanavalin A (Con A; 0.5-10μg/ml). When whole and MO-depleted splenocytes were preincubated with YE (2μg/ml) for 24h, the prolifera-tion of whole splenocytes was significantly enhanced compared to that of whole splenocytes preincubated with medium alone. In contrast, MO-depleted splenocytes did not show any increase of proliferation following in vitro pretreatment with VE. When the splenic MO pretreated with both YE (2μg/ml) and Con A (10μg/ml) for 24h were further incubated with splenic lymphocytes, their proliferation was significantly enhanced compared to that of splenic lymphocytes cultured with splenic MO pretreated with Con A. In this experiment, the medium containing 2-mercapto-ethanol (2-ME) had the ability to enhance splenic lymphocyte prolifera-tion, which masked the enhanced effect of YE on splenic lymphocyte proliferation. Furthermore, in vitro treatment of VE could not decrease the production of prostaglandin E2, but could enhance the production of interleukin 1 from splenic MO. These results suggest that MO play an important role in the proliferation of splenic lymphocytes following in vitro incubation with VE, which is closely associated with the action of VE as an immunomodulator rather than antioxidant.