DEVELOPMENT AND VALIDATION OF A RAPID AND SIMPLE REVERSED-PHASE HPLC METHOD FOR THE DETERMINATION OF GEMCITABINE IN HUMAN PLASMA

ABSTRACT Objective: Mannich bases of 2-naphthol are predominantly popular in metal-mediated and ligand-accelerated catalysis of enantioselective carbon-carbon bond formation. Since these compounds have multiple centre for chelation with metal ions, they are likely to be potent inhibitors of metallo-enzymes. A number of pharmaceutical and agricultural agents have a naphthalein frame work. Our present study focuses on the synthesis of Mannich base derived from the condensation of 2-naphthol, benzaldehyde and thiourea and its metal complexes and their biological activities. Methods: The ligand 1-(naphthalein -2-yloxy )(phenyl)(methyl) thiourea (BNBTU) was synthesized by Mannich condensation reaction  between 2- naphthol, benzaldehyde and thiourea in 1:1:1 molar ratio. Mn(II), Co(II), Ni(II), Cu(II)  and Zn(II) complexes of the new Mannich base BNBTU have been synthesized. Results: The anti-bacterial activity of the ligand and all the metal complexes leads to the conclusion that most of the complexes were found to have activities against E.coli and B. subtilis . The cytotoxic effects of the newly synthesized ligand have been found good inhibition activity against the cancer cell line. Further the ligand and the metal complexes have been screened for their fungicidal and anti-oxidant properties and they are found to be significantly active. Conclusion: The ligand 1-(naphthalein -2-yloxy )(phenyl)(methyl) thiourea (BNBTU) has shown as one of the novel ligand and its coordination with transition metals exhibited enhanced biological activity. Key words : Mannich base, Spectral studies, Antimicrobial activity and Anti-cancer activity.