The inhibition of accessory gene regulator C specific binding peptides on biofilm formation of Staphylococcus epidermidis on the surface of polyvinyl chloride in vitro

目的: 探讨表皮葡萄球菌附属基因调节子 C（accessory gene regulator C，agr C）特异结合多肽（简称为 N1）对聚氯乙烯（polyvinyl chloride，PVC）材料表面表皮葡萄球菌生物膜形成作用的体外研究。. 方法: 以表皮葡萄球菌 ATCC35984（生物膜表型阳性）和 ATCC12228（生物膜表型阴性）作为研究对象。首先将两菌株分别加入浓度为 100、200、400、800、1 600 μg/mL 的 N1 培养，以相同浓度 agrC 特异结合无关肽（简称为 N0）培养作为对照；24 h 后测定吸光度（ A）值，确定 N1 最佳抑菌浓度。两菌株与最佳抑菌浓度的 N1 及 N0 分别培养，6、12、18、24、30、48 h 测定 A 值，观察 N1 对表皮葡萄球菌生物膜形成能力的影响；在此基础上与 PVC 材料片培养 6、12、18、24、30 h，扫描电镜观察 PVC 材料表面生物膜的表面结构；另取与 ATCC35984 菌株培养 6、12、18、24 h 的 PVC 材料片，激光共聚焦显微镜观察生物膜厚度。. Results: The optimal bacteriostatic concentration of N1 was 800 μg/mL. ATCC 12228 strain did not form obvious biofilm after being cultured with N1 and N0. When ATCC35984 strain was cultured with N1 and N0 for 12 hours, the difference in biofilm formation ability between groups N1 and N0 was statistically significant ( P 0.05). Scanning electron microscopy examination showed that mature biofilm structure was observed in ATCC35984 strain and was not observed in ATCC12228 strain. Laser confocal microscopy observation showed that the number of bacteria in the group N1 was significantly lower than that in the group N0 at 12 hours, and the most of bacteria were dead bacteria. There was no significant difference in the number of bacteria at 6, 18, and 24 hours, and the most of them were live bacteria. The biofilm thickness of group N1 was significantly lower than that of group N0 at 12 and 18 hours ( P<0.05). 结论: N1 抑制表皮葡萄球菌生物膜形成的强度有量效关系；其在聚集期阻碍细菌的增殖和聚集，抑制生物膜形成，对成熟生物膜抑制作用不明显。.