Toxicidad crónica de polvo de taninos obtenidos de corteza de Pinus caribaea Morelet por vía oral en ratas

INTRODUCCION: se han demostrado los efectos antimutagenicos y no genotoxicos de los taninos de Pinus caribaea Morelet, asi como su influencia en la mejoria de la calidad de vida de pacientes. OBJETIVOS: evaluar la toxicidad cronica del polvo de taninos obtenido de corteza de P. caribaea por via oral. METODOS: se realizo un estudio de toxicidad cronica (6 meses) por via oral en ratas Cenp:SPRD. Se conformaron un grupo control y 3 tratados (1, 2,5 y 5 mg/kg/d/6 meses). Se realizaron observaciones clinicas diarias. El peso corporal y los consumos de agua y alimento se midieron semanalmente. Al inicio, en la semana 13 y al final del estudio se realizaron examenes de laboratorio clinico. Al concluir los 6 meses se sacrificaron todos los animales y se efectuo necropsia completa de cada uno, asi como el estudio microscopico de los organos y tejidos de los animales de los grupos Dosis alta y control. RESULTADOS: durante el estudio murio 1 hembra del gurpo Dosis baja, el resto de los animales alcanzo el final del estudio con buen estado. Los signos clinicos descritos estuvieron distribuidos por todos los grupos y no guardaron relacion con la sustancia de ensayo. La curva de peso corporal fue similar para todos los grupos. El consumo de agua se incremento en los animales tratados con la dosis mayor, el consumo de alimento no se vio afectado. Los parametros de laboratorio clinico presentaron diferencias, aunque se mantuvieron dentro de los rangos normales reportados para la especie. Los hallazgos de anatomia patologica no guardaron relacion con la administracion del polvo de taninos. CONCLUSIONES: la administracion oral durante 6 meses del polvo de taninos de corteza de P. caribaea no produjo toxicidad. INTRODUCTION: effects of antimutagenic and non-genotoxic agents of tannins from Pinus caribaea Morelet, as well as its influence in improvement of quality life of patients. OBJECTIVES: to assess chronic oral toxicity of tannin powder obtained from bark of P. caribaea. METHODS: we performed a study on chronic toxicity (6 months) per os in Cenp:SPRD rats. We made clinical observations daily. There was a control group and 3 treated (1, 2, 5 and 5 mg/kg/d/6 months). We performed clinical observations daily. Body weight and water and food consumptions were measured weekly. At the beginning, in 13 week and at the end of study, we carried out clinical laboratory examinations. At 6 months all animals were scarified and we made a complete necropsy of each, as well as a microscopical study of organs and tissues of animals in groups of higher dose and control. RESULTS: during study a female in lower dose group died, the remainder finished the study in a good sate. Clinical signs described were distributed by all groups and had not relation with assay substance. Body weight curve was similar for all groups. Water consumption increased in animals treated with the higher dose, but the food consumption was normal. Clinical laboratory parameters had differences, although they were within normal ranks reported for the species. Findings of pathologic anatomy have not relation to administration of tannin powders. CONCLUSIONS: There was not toxicity with per os administration during 6 months of tannin powders from bark of P. caribaea.