Simultaneous Determination of Four Medicinal Components in Lamiophlomis rotate

To establish a HPLC method for determination of forsythoside B,acteoside,luteoloside and luteolin in L.rotate,the determination method was established by using methanol extracts fromL.rotate sample,the linearity range and average recovery rate of them were analyzed.The results showed that four medicinal components,forsythoside B,acteoside,luteoloside and luteolin,were obtained in L.rotate sample;Degree of precision,RSD of the four medicinal components was 0.65%,0.92%,0.73% and 0.77%respectively.Stability,RSD was 0.78%,1.15%,1.06% and 1.22% separately;RSD was 1.35%,0.76%,1.18%and 0.91%respectively.The recovery rates of the four medicinal components were 100.66%,99.32%,97.07%and 99.65% with RSDof 2.83%,1.97%,2.01%and 1.58%respectively.The contents of the four medicinal components of the ten groups of medicinal materials were respectively 0.430~6.782mg/g,0.661~8.600mg/g,1.320~6.877mg/g and 0.043~0.154mg/g respectively.This method could be used for simultaneous determination of forsythoside B,acteoside,luteoloside and luteolin in L.rotate for its accuracy,reliability and good repeatability.