Study on Callus Induction fromin vitro Capsicum annuum L.

In order to use callus to regenerate plant and do genetic transformation,taking two varieties of Capsicum annuum L.seeds as materials which were used to induce in vitro plantlets(Capsicum annuum L.),and taking hypocotyls of different seedling age as explants,the differences between their induction effect were compared,and the induction effect of different concentrations combination of IAA and 6-BA on callus were discussed.The results showed that the best seedling age to induce callus was at 16das explant callus.The best medium for callus induction by hypocotyls was MS medium supplemented with 0.8mg·L-1 IAA,1.0mg·L-1 6-BA and 4.0mg·L-1 AgNO3,and the effect of callus induction by Pepper king was better than Japanese pod pepper,it showed that genotype had highly significant impact on induction of callus fromin vitro Capsicum annuum L..