Simultaneous determination of pancuronium, vecuronium and their related compounds using LC–ESI-MS
2006
Abstract A simultaneous determination method of quaternary amino steroidal muscle relaxants, pancuronium (PAN), vecuronium (VEC), and 17-monodesacetyl pancuronium (17-OH-PAN), 3,17-bisdesacetyl pancuronium (3,17-OH-PAN), 3-monodesacetyl vecuronium (3-OH-VEC), 3,17-bisdesacetyl vecuronium (3,17-OH-VEC) in human serum was developed using liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS). The weak cation exchange cartridge was useful for the extraction of these compounds. Under optimized LC–ESI-MS conditions, these compounds were almost fully separated within 6.5min. Linear responses over the concentration range 0.25–50.0ng/mL were demonstrated for each compound. The developed method successfully detected VEC, 3-OH-VEC and 3,17-OH-VEC in serum intravenously administered with VEC. The level of 3-OH-VEC was higher than other compounds. This suggested that 3-OH-VEC was useful as a forensic probe in VEC administration.
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