Correlation analysis of microsatellite DNA markers with growth related traits of swimming crab（ Portunus trituberculatus）

In this study,total 35 microsatellite DNA markers were selected to analyze the genetic structure of 110 individuals in an F 2 family which is obtained by directional mating techniques for the selective program on fast growing Portunus trituberculatus.(The F 2 family was created by F 1 family which was generated by wild populations from Laizhou Bay and Zhoushan Island.) The results showed that total 87 different alleles were found,and the number of alleles was 2 to 4 in each locus,with 2.2 effective alleles on average.The value of mean observed heterozygosity,the value of mean expected heterozygosity and the mean Polymorphism Information Content(PIC) was 0.646 5,0.513 0 and 0.449 1,respectively.The probability value of chi-square test showed that most of the loci deviated from Hardy-Weinberg equilibrium significantly.The GLM procedure in SAS9.1 was used to analyze the correlation between these 35 microsatellite markers and growth related traits of P.trituberculatus.The growth related traits included full carapace width,body weight,carapace width,carapace length,body height,second obital margin width,meropodit length of the claw,meropodit length of the first peraeopod.Results indicated that Pot08 had a significant impact on full carapace width,body weight,carapace length,body height,second obital margin width;Pot42 had a significant impact on body weight,carapace length,meropodit length of the claw,meropodit length of the first peraeopod;Pot53 had a significant impact on carapace width and meropodit length of the claw,and had a very significant impact on second obital margin width;Pot57 and PTR30 had a significant impact on meropodit length of the first peraeopod and carapacel length,body weight,respectively;PTR8a had a significant impact on the first peraeopod and carapace length,body height,and had a very significant impact on body weight,second obital margin width,full carapace width and carapace width;PTR131 had a significant impact on body weight,full carapace width and carapacel width.Finally,PTR8a can be used for marker assisted selection which could be used for body weight selection.