Determination of anion content in polysaccharide products by ion chromatograph

Ash method,acidic hydrolysis and online ultrafiltration pretreatment methods were introduced into anionic contents ion chromatographic analysis of Cl-,SO4 2-,PO4 3-in polysaccharide products. A quick and convenient method has been developed for simultaneously determining the anion contents,such as Cl-,SO4 2-,PO4 3-in polysaccharide products. This method could realize the separation and detection of the anions simultaneously using sodium carbonate,sodium bicarbonate and acetone as mobile phase and inline filtration in pretreatment of the polysaccharide products. The calibration curves showed good linearity. The detection limits( LOD,S/N=3) of Cl-,SO4 2-,PO4 3-were 0. 03,0. 05 and 0. 08μg·mL-1. By spiked standard solutions in the samples,the recoveries were in the range of 96. 2% ~ 103. 6%. The peak areas showed good repeatability. The method has a good reliability and practicability with high sensitivity,high accuracy,good stability,low detection limit,simple sample pretreatment,simple and quick operation.