The effect of goat milk yoghurt casein antioxidant activity on histopathology of lung in male Rattus norvegicus exposed by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Dioxins are highly toxic and carcinogenic compounds. The most dangerous dioxin is 2,3,7,8-tetrachloro-dibenzo-p-diozin (TCDD). TCDD dioxin can cause health problem accumulates overtime. Goat milk yoghurt casein can reduce free radicals caused by TCDD. Goat milk yoghurt casein contains bioactive peptide that acts as antioxidant. The aim of the current research was to determine the effect of goat milk yoghurt casein towards lung histopathology after TCDD exposure. This research was an experimental study using a completely randomized design (CRD). Male Rattus norvegicus were divided into 5 groups: K- (negative control), K+ (positive control induced by TCDD 100 mg/kgBW), K1 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 300 mg/kgBW), K2 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 600 mg/kgBW), and K3 (given TCDD 100 mg/kgBW and goat milk yoghurt casein 900 mg/kgBW). Lung were collected for histopathological examination with scoring methods such as vascular congestion, thickening of septa alveoli, and focal necrosis around blood vessels. Quantitative analysis with Kruskal Wallis and Mann-Whitney test showed that goat milk yoghurt casein could prevent lung damage due to TCDD exposure. It can be concluded that goat milk yoghurt casein 900 mg/kgBW can prevent lung damage such as vascular congestion, thickening of septa alveoli, and focal necrosis around blood vessels. Thus, goat milk yoghurt casein could be used as antioxidant source against TCDD exposure.