Expression of Cyt-C, Caspase 3, Caspase 8 in auditory cortex after bilateral cochlear ablation in rats

OBJECTIVE To research the expression of Cyt-C,Caspase 3 and Caspase 8 in auditory cortex after bilateral cochlear ablation in rats.METHODS 48 SD rats were randomly divided into 2-week group,4-week group,6-week group,8-week group and 4 corresponding control groups(CG)with 6 rats(n=12)in each group.Bilateral cochlear ablation was done in experiment groups and then immunohistochemical detection was adopted for Cyt-C,Caspase 3 and Caspase 8 in bilateral auditory cortex of each group.RESULTS The model of auditory deprivation on rat was successfully established.The expression of Cyt-C increased by time,with statistical differences(P 0.05)between any two traumatized groups.The expression of Caspase 3 and Caspase 8 increased by time too,but with statistical differences(P0.05)only between 2-week and 6-week,2-week and 8-week,4-week and 6-week,4-week and 8-week,6-week and 8-week.CONCLUSION The speed of neuronal apoptosis in auditory cortex caused by bilateral cochlear ablation was slow before 4 weeks and fast after 6 weeks.