Abstract 4935: A quantitative model to study cell-type-specific transcriptional regulation between MCF-7 and LNCaP

Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL Transcriptional regulation lies at the heart of biological processes. To further understand the regulatory mechanism, we are studying the transcriptional networks of nuclear receptors in breast cancer cell line MCF-7 and prostate cancer cell line LNCaP. Based on whole-genome gene expression microarray data, we observed very different transcriptional programs induced by ligands of nuclear receptors between these two cell lines. In order to systematically explain cell-type-specific transcriptional regulation, we are trying to build a quantitative model to predict gene expression in different cellular contexts under different ligand stimulations. The model will be trained using data including genomic binding sites of nuclear receptors (ChIP-Seq), and time-series expression of nuclear receptors, cofactors and their target genes (Nanostring). The output of the model will be gene expression trajectories after ligand treatments in the two cell lines. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4935. doi:1538-7445.AM2012-4935