Association between TP53 Codon 72 Single-Nucleotide Polymorphism and Radiation Sensitivity of Human Fibroblasts

Abstract Alsbeih, G., Al-Harbi, N., Al-Buhairi, M., Al-Hadyan, K. and Al-Hamed, M. Association between TP53 Codon 72 Single-Nucleotide Polymorphism and Radiation Sensitivity of Human Fibroblasts. Radiat. Res. 167, 535–540 (2007). Inherent radiosensitivity varies widely between individuals. We hypothesized that amino acid substitution variants in two highly radiation-responsive proteins, TP53 (p53) and CDKN1A (p21, Waf1, Cip1), are associated with and could explain individual variations in radiosensitivity. The two non-synonymous single-nucleotide polymorphisms (SNPs) TP53 codon 72 Arg/Pro G>C and CDKN1A codon 31 Ser/Arg C>A were genotyped in 92 normal fibroblast cell strains of different radiosensitivity. The clonogenic surviving fraction at 2 Gy (SF2) ranged between 0.15 and 0.50 (mean = 0.34, SD = 0.08). The mean SF2 was used to divide the cell strains into radiosensitive (45) and normal groups (47). A significant association was observed between SF2 and the TP53 codon 72 haplotype (C compared to G, P =...