Acid-sensitive connects the synthesis of unit and the purposes in DNA sequencing thereof

The invention discloses a kind of acid-sensitive and connect the synthesis of unit and the purposes in DNA sequencing thereof.This acid-sensitive connects unit, and structural formula is: Wherein R is NH 2 Or N 3 , m is any integer in 0～44, and n is any integer in 0～44；R 1 , R 2 It is aliphatic alkyl, or R 1 , R 2 It is aromatic derivant, or R 1 For phenyl, naphthyl, the derivant of phenyl or the derivant of naphthyl, R 2 For aliphatic alkyl or hydrogen；Or R 2 For phenyl, naphthyl, the derivant of phenyl or the derivant of naphthyl, R 1 For aliphatic alkyl or hydrogen, or R 1 、R 2 Constitute cyclohexyl, cyclopenta or cyclobutyl.This acid-sensitive connection unit is connected the Reversible terminal obtained and can be used for DNA synthesis order-checking with nucleotide and fluorescein.Such Reversible terminal can be used for DNA sequencing；Meanwhile, its synthesis needed raw material is simple and easy to get, and building-up process is conventional chemical reaction, can be used for large-scale promotion and uses.