Isolation and Resistance of Lactobacillus from Intestinal Mucosa of Bluntnose Black Bream(Megalobrama amblycephala)

Two strains of Lactobacillus JK-1 and JK-2 were isolated from intestinal mucosa of bluntnose black bream(Megalobrama amblycephala)which showed an effective inhibition to Aeromonas hydrophila.A tolerance test was conducted for cholate,pH,protease,and high temperature.Among the cholate-tolerance test,over 35% of JK-1 and JK-2 survived after culturing for 4 and 0.4% concentration in cholate.And in pH-tolerance test,two strains of lactic acid bacteria survived in both acid and alkaline environment.Trypsin did not affect the their survival.Moreover,they performed certain tolerance to the megatemperature of 70 ℃.