Dibutyl phthalate induced oxidative stress and genotoxicity on adult zebrafish (Danio rerio) brain.

Abstract Dibutyl phthalate (DBP) is one of the most widely used plasticizers with a high concentration in the water. Although the toxicity of DBP on aquatic organisms has become a significant concern in recent years, the effects of DBP on zebrafish (Danio rerio) brain is poorly understood. This study investigated the toxic effects of DBP exposure for 7, 14, 21 and 28 days on zebrafish brain. The results showed that DBP significantly stimulated SOD and CAT activities, increasing MDA and 8-OHdG contents. On the 28th day, the AChE inhibition rates in 0.08, 0.4, 2 mg·L-1 treatment were 13.4%, 11.9%, 14.7%. The trend of Cu/Zn-sod gene variation was consistent with SOD activity, showing “inhibition-activation-inhibition”. The expression of apoptotic genes (caspase-3, p53) showed “inhibition-activation-inhibition”. The integrated biomarker response (IBR) results showed that the IBR values were 4.37, 7.18 and 9.63 in 0.08, 0.4 and 2 mg·L-1 group on the 28th day, presenting a “dose-response” relationship. These findings confirmed that low concentration of DBP induced oxidative damage and genotoxicity in zebrafish brain, which provided an effective toxicological basis for phthalate pollution. Based on above studies, it is of great significance for assessing the harmful effects of DBP with low concentration on aquatic organisms.