Identification and detection of Dermatophagoides farinaand Dermatophagoides pteronyssinus by Polymerase Chain Reaction

The purpose of the present study was to identify and detect Dermatophagoides farina(D.f) and Dermatophagoides pteronyssinus(D.p)by Polymerase Chain Reaction(PCR) technology.A randomly primed PCR(RP-PCR) technology was used to amplify the DNA of D.f and D.p,and three amplified bands were selected for DNA sequencing and analysis.According to the resuLts of DNA sequencing,another two pairs of primers were designed to identify and detect D.f and D.p,then the specificity and sensitivity of these primers were analyzed.In amplification of RP-PCR,two significant bands around 500bp and 750bp were observed in amplified products of D.f,while only a clear band about 500bp in D.p and no product larger than 500bp appeared.Three new DNA fragments of D.f and D.p were sequenced and analyzed in homology.As the new primers designed were highly specific and sensitive in detection of the two mites,RP-PCR technology could be used to identify D.f and D.p.Results indicate that the PCR method verified in the research has a potentiality to detect D.f and D.p in the environment.