Effects of aquatic conditioning on cartilage and bone metabolism in young horses

While beneficial in rehabilitation, aquatic exercise effects on cartilage and bone metabolism in young, healthy horses has not been well described. Therefore, thirty Quarter Horse yearlings (343 ± 28 kg; 496 ± 12 d of age) were stratified by age, body weight (BW), and sex and randomly assigned to one of three treatments for 140-d to evaluate effects of aquatic, dry, or no exercise on bone and cartilage metabolism in young horses transitioning to an advanced workload. Treatments included non-exercise control (CON; n=10), dry treadmill (DRY; n=10), or aquatic treadmill exercise (H2O; n=10; water: 60% wither height, WH). Horses were housed individually (3.6×3.6 m) from 0600 to 1800, allowed turnout (74×70 m) from 1800 to 0600, and fed to meet or exceed requirements. During Phase I (d 0-112), DRY and H2O walked on treadmills 30 min/d, 5 d/wk. Phase II (d 113-140) transitioned to an advanced workload 5 d/wk. Every 14-d, WH, hip height (HH), and BW were recorded. Left third metacarpal radiographs on d 0, 112, and 140 were analyzed for radiographic bone aluminum equivalence (RBAE). Every 28-d, serum samples were analyzed for osteocalcin and C-telopeptide crosslaps of type I collagen (CTX-1), and synovial fluid samples were analyzed for prostaglandin E2 (PGE2), collagenase cleavage neopeptide (C2C), collagenase of type I and type II collagen (C1,2C), and carboxypeptide of type II collagen (CPII) using ELISAs. All data were analyzed using PROC MIXED of SAS, including random effect of horse within treatment, and repeated effect of day. Baseline treatment differences were accounted for using a covariate. There were treatment × day interactions (P 0.30) in RBAE, BW, or HH, but all increased over time (P<0.01). There were no treatment × day interactions of synovial inflammation or markers of cartilage metabolism, however there was an effect of day for each marker (P<0.03). Changes in biomarkers of cartilage turnover in horses exercised at the walk, whether dry or aquatic, could not be distinguished from horses with access to turnout alone. This study indicates that early forced exercise supports consistent bone metabolism necessary for uniform growth and bone development, and that there are no negative effects of buoyancy on cartilage metabolism in yearlings transitioned from aquatic exercise to a 28-d advanced workload.