Visualizing endocytic recycling and trafficking in live neurons by subdiffractional tracking of internalized molecules

Merja Joensuu,Ramón Martínez-Mármol,Pranesh Padmanabhan,Nick R. Glass,Nela Durisic,Matthew Pelekanos,Mahdie Mollazade,Giuseppe Balistreri,Rumelo Amor,Justin J. Cooper-White,Geoffrey J. Goodhill,Frederic A. Meunier

Visualizing endocytic recycling and trafficking in live neurons by subdiffractional tracking of internalized molecules

2017

Merja Joensuu
Ramón Martínez-Mármol
Pranesh Padmanabhan
Nick R. Glass
Nela Durisic
Matthew Pelekanos
Mahdie Mollazade
Giuseppe Balistreri
Rumelo Amor
Justin J. Cooper-White
Geoffrey J. Goodhill
Frederic A. Meunier

This protocol describes a pulse–chase approach to studying activity-dependent internalization of fluorescent ligands into endocytic compartments using subdiffractional single-particle tracking in live hippocampal neurons.

Keywords:

Cell biology
Internalization
Endocytic recycling
Hippocampal formation
Biology
Endocytic cycle
Nervous system
Synaptic vesicle
Single-particle tracking

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