Validation Study on Five Cytotoxicity Assays by JSAAE - VI. Precise Results of the LDH Release Assay

The inter-laboratory validation study on 5 cytotoxicity assays conducted by JSAAE has been described in the preceding articles (see Validation Articles I V). Presented here are precise data and the protocol of the LDH release assay with two cell lines, HeLa S3 (SC) that is common to the other 4 assays and SQ-5 that is rich in LDH activity. The performance rates in each of the four LDH-release sub-divided assays were extremely low. We concluded that the present LDH release assay is practically immature, although the assay is very informative in principle not only on the cell growth inhibition but also on the cell lysis caused by the tested chemicals.