Effects of nicotine on bone marrow stromal cells proliferation and differentiation of chondrocyte in vitro

Objective:To examine the effects of various concentration of nicotine on bone marrow stromal cells(BMSCs) proliferation and differentiation of cartilaginous in vitro.Methods:BMSCs was obtained from femoral bone and tibia of New-Zealand albino rabbit.The cells of the 3rd generation were used in study.Different concentration of nicotine(0,1×10-7,1×10-6,1×10-5 M) were added into BMSCs.BMSCs proliferation was analyzed by MTT assay at the 1,4,7,14 days.The expression of collagen typeⅡand aggrecan as the marker genes of cartilaginous differentiation from BMSCs were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results:Microscope showed that BMSCs transformed from round to fusiform shape.The concentration of nicotine in 1×10-7,1×10-6 M had a significant positive effect on cell proliferation and the expression of typeⅡcollagen in a time-dependent manner when supplemented in commonly used induction media(P0.05).Concentrations of nicotine in 1×10-7 can promote the expression of aggrecan at the 7th day after induction,and in 1×10-5 M may inhibit the expression of typeⅡcollagen and aggrecan.Conclusion:It was implied that local application of nicotine at an appropriate concentration may be a promising approach for enhancing cartilaginous differentiation capacity of BMSCs in cartilage tissue engineering.