Inhibitory effect of Dexosome from dendritic cells treated with heat-CO2 pneumoperitoneum of proliferation of human gastric adenocarcinoma AGS cells

Objective To observe the inhibitory effects of dendritic cells (DCs)-derived Dexosome (Dex) on proliferation of human gastric adenocarcinoma cell line AGS after treated with heat-CO2 and investigate the value of heat-CO2 pneumoperitoneum used during laparoscopic surgery.Methods An in vitro heat-CO2 pneumoperitoneum experimental box was built.After heat-CO2 pneumoperitoneum treatment,the DCs-derived Dex was isolated and identified through transmission electronic microscope (TEM) and Western blotting.AGS cells were co-cultured with Dex,and the cell proliferation was measured by cell counting kit-8 (CCK-8).The expression of heat shock protein 70 (HSP70) in Dex was examined by Western blotting.Results The Dex from DCs culture supernatants was revealed to be 30 to 100 nm membrane vesicles by TEM,and its expression of marker CD63 was significantly higher than whole cell lysates (P ＜0.05).The proliferation inhibition rate of AGS cells was (20.50 ± 3.07) ％ in heat treated group and (45.30 ±2.13)％ in heat-CO2 treated group respectively (P ＜ 0.05).The expression of HSP70 in Dex treated with heat-CO2 was significantly higher (P ＜ 0.05).Conclusion Dex was isolated successfully.Proliferation of AGS cells could be inhibited significantly by Dex after DCs treated through Heat-CO2,which may be related to the over-expression of HSP70 molecules in Dex. Key words: Heat-CO2; Dendritic cell; Gastric adenocarcinoma; Proliferation inhibition