Receptor-induced Ca2+ signaling in cultured myenteric neurons

We studied the effect of excitatory neurotransmitters (10− 5 M) on the intracellular Ca2+ concentration ([Ca2+]i) of cultured myenteric neurons. ACh evoked a response in 48.6% of the neurons. This response consisted of a fast and a slow component, respectively mediated by nicotinic and muscarinic receptors, as revealed by specific agonists and antagonists. Substance P evoked a [Ca2+]i rise in 68.2% of the neurons, which was highly dependent on Ca2+ release from intracellular stores, since after thapsigargin (5 μM) pretreatment only 8% responded. The responses to serotonin, present in 90.7%, were completely blocked by ondansetron (10− 5M), a 5-HT3 receptor antagonist. Specific agonists of other serotonin receptors were not able to induce a [Ca2+]i rise. Removing extracellular Ca2+ abolished all serotonin and fast ACh responses, whereas substance P and slow ACh responses were more persistent. We conclude that ACh-induced signaling involves both nicotinic and muscarinic receptors responsible for a fast and a...