Multiplexed High Content Assays for Predictive Hepatotoxicity using Induced Pluripotent Stem Cell Derived Hepatocytes

Multi-parametric Image Analysis can be used to monitor changes in cell viability (Calcein AM), nuclear shape (Hoechst), and mitochondria potential (MitoTracker Orange) associated with different types of toxicity. The ImageXpress Micro system allows automatic analysis on a cell-by-cell basis using multi-wavelength cell scoring. The SpectraMax Paradigm plate reader can also be used to determine toxicity response on a well-by-well basis.