Effect of NaF on proliferation and mineralization of human periodontal ligament cells

PURPOSE: To investigate the effect of NaF on proliferation and mineralization of human periodontal ligament cells (hPDLCs). METHODS: hPDLCs were isolated and characterized. The proliferation of hPDLCs treated with different concentration of NaF was tested by CCK-8. Four moderate concentrations were chosen for subsequent experiments. The mineralization was investigated using ALP activity assay, Alizarin red S staining and quantitative real-time polymerase chain reaction(RT-PCR). The data were statistically analyzed with SPSS20.0 software package. RESULTS: Immunohistochemistry showed that the isolated cells were hPDLCs. 5×10-5, 1×10-4 and 5×10-4 mol/L NaF had pro-proliferation effects while 5×10-4 mol/L resulted in the best effect (P<0.05). ALP activity and calcium content was significantly enhanced by 1×10-5 mol/L NaF with osteogenic inductive medium (P<0.05). Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. CONCLUSIONS: 5×10-5, 1×10-4, 5×10-4 mol/L NaF can stimulate proliferation in hPDLCs, 1×10-5 mol/L NaF can enhance ALP activity and calcium content formation of hPDLCs.