74. Effect of acute stress on T cells and plasmatic cells IgA+ in Peyer’s patches of duodenum and ileum of BALB/c mice

Stress is considered a disease of our time, affecting a number of people every day, people usually do not understand the stress as something that affects your health. Studies on the effect of stress on the immune system specifically in animal models have shown that the effect varies depending on the type of stress, its duration and if applied acutely or chronically. Changes in cell percentages of different cell populations produced by the application of restraint stress protocol for acute motion has been studied as a reference and in parallel to other parameters such as the effect of hormones, but has not paid sufficient attention to these changes. The aim of this work is to establish changes in the percentages of the populations of LB (B220/CD19), T CD4+ and TCD8+ in Peyer’s patches proximal and distal both during the state of acute stress-induced restriction of movement and during the subsequent recovery status of 2 h. Populations were studied by flow cytometry and corticosterone was measured by enzyme immunoassay (EIA). Flow cytometry showed that CD4+ T, B cells and plasmatic cells IgA+ increased in the stressed animal in Peyer’s patches. Actual changes were observed in lymphocyte populations, but to know the nature of these is necessary for future work studying the degree of apoptosis and the expression of integrins. This work was supported by SIP-COFAA-IPN.