An efficient wound healing hydrogel based on a hydroalcoholic extract of Moringa oleifera seeds

Abstract Objective The study aimed to assess the efficacy of formulated wound healing hydrogel based on a hydroalcoholic extract of Moringa oleifera seeds. Methods In vitro antioxidant (using DPPH method) and antimicrobial activity (using agar well diffusion method) was studied. Male Swiss albino mice of weight 20- 30g were used. The hydrogel containing 5% and 10% of hydroalcoholic extract of Moringa oleifera seeds was used, whereas Povidone-iodine (5%) was used as standard. The wound healing efficacy of formulated hydrogel based on a hydroalcoholic extract of Moringa oleifera seeds was studied using excision and incision wound models. Assessments were taken on the 1st, 4th, 6th, 9th, 12th, 13th and 14th day in the excision model and on the 8th day in the incision model. Histopathological assessments were also done to support the study. Results Hydroalcoholic extract of Moringa oleifera seeds showed significant scavenging of free radicals showing antioxidant activity. Plant extract showed significant antimicrobial activity using the agar well diffusion method. The formulated hydrogels using the hydroalcoholic extract of Moringa oleifera seeds showed significant wound healing activity compared to both control (p Conclusion The study presented here proved the hydrogels formulated using the hydroalcoholic extract of Moringa oleifera seeds could be used as an alternative therapy to manage wounds effectively.