Inhibition of fibroblast histomorphology induced by sonodynamic therapy with hematoporphyrin monomethyl ether

Objective To investigate the mechanism of the effect of HMME-SDT on the proliferation of hypertrophic scar fibroblasts (Fb). Methods Fibroblast suspensions of rabbit ear with hypertrophic scar were divided into four groups: control group, ultrasound group, HMME group and HMME-SDT group. The survival of the fibroblast suspension of rabbit ear were determined by MTT assay method. The mitochondrial membrane potential, cellular reactive oxygen, the concentration changes of cellular calcium ion were also investigated by flow cytometry correspondingly. Nucleolar morphology was observed by 33258 nucleoli stain. Results There was significant difference between the SDT group and control, HMME and ultrasound groups at same time (P< 0.01). There were significant differences between the HMME-SDT group and HMME, ultrasound and control groups on cellular reactive oxygen, nucelus concentration and floccuent change as well as nuclear margination. Conclusions HMME-SDT can inhibit the proliferation of hypertrophic scar fibroblasts of rabbit ear significantly. Key words: Sonodynamic therapy; Hematoporphyrin monomethyl ether; Fibroblast