Differentiation of cardiomyocytes and generation of human engineered heart tissue

Kaja Breckwoldt,David Letuffe-Brenière,Ingra Mannhardt,Thomas Schulze,Bärbel M. Ulmer,Tessa Werner,Anika Benzin,Birgit Klampe,Marina Reinsch,Sandra Laufer,Aya Shibamiya,Maksymilian Prondzynski,Giulia Mearini,Dennis Schade,Sigrid Fuchs,Christiane Neuber,Elisabeth Krämer,Umber Saleem,Mirja L. Schulze,Marita L Rodriguez,Thomas Eschenhagen,Arne Hansen

Differentiation of cardiomyocytes and generation of human engineered heart tissue

2017

This protocol describes how to generate defined embryoid bodies and subsequent standardized beating engineered heart tissue from human iPSCs using small molecules.

Keywords:

Cell biology
Induced pluripotent stem cell
Molecular biology
Tissue engineering
Small molecule
Cellular differentiation
Biology
Embryoid body
Cell culture
Contractility
Myocyte
Growth factor

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