Human Osteoblast–Derived Factors Induce Early Osteogenic Markers in Human Mesenchymal Stem Cells

The capacity of human mesenchymal stem cells (hMSC) for self-renewal and differentiation is a tightly regulated process within their microenvironment—the stem cell niche. For future therapeutic applications of hMSC within the frame of tissue engineering, it is of major importance to understand the factors involved in triggering differentiation cascades of hMSC. Using either osteoblast-conditioned medium or an indirect coculture system, we investigated whether soluble factors from human osteoblasts (hOB) are sufficient to induce early osteogenic markers in hMSC. Thereby, we detected an induction of several osteogenic markers like alkaline phosphatase, bone sialoprotein 2, leptin receptor, decorin, and cathepsin K in hMSC as indicators of the onset of early osteogenesis. Further, because Wnt signaling has been reported to play an important role in osteogenesis, we performed RNAi against the main Wnt mediator β-catenin and the low-density lipoprotein receptor-related protein 5 as a major Wnt co-receptor in h...