Physiology of Vero, CHO and Hybridoma Cells Cultured in Bioreactor

Objective To study on the growth and metabolism of Vero, CHO and hybridoma 7H3 cell lines cultured in bioreactor. Methods Vero, CHO and 7H3 cells were cultured in stirring bioreactor. The nutrient and metabolic product were quantitatively analyzed, and fermentation-related parameters were detect-ed . Results The intakings of gulcose and glutamine during culture were positively related to the concentra-tions of them in culture fluid, and the yield of cells could be increased by controlling the addition of nutrient. Conclusion Vero, CHO and 7H3 cells show the same modes of growth and metabolism in bioreactor.