Semiquantitative analysis of urinary low protein levels using silver dot blot assay

We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 μl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining reagent, and the optical density of the silver stained urinary protein is measured at 500 nm using a densitometer. There was a good linearity between 2.5 mg/L and 100 mg/L and a gentle linearity between 100 mg/L and 200 mg/L, and the minimum sensitivity was 2.5 mg/L. This method is suitable for semiquantitative analysis of urinary protein levels less than 300 mg/L, which can not be determined precisely by dipstick. J. Clin. Lab. Anal. 15:171–174, 2001. © 2001 Wiley-Liss, Inc.