Development of Monoclonal Hybridoma Cell Lines and Extracting Antibody Against Fummonisin B1

Objective : To acquire monoclonal hybridoma cell lines against fummonisin B 1 (FB 1 ) and extract monoclonal antibody against FB 1 . Methods : Coupling antigens of FB 1 -KLH and FB 1 -BSA with chemical methods and immune 6-8 weeks old female BALB/c mice with FB 1 -KLH. Integrating spleen cells with sp2/0 myeloma cells to acquire hybridoma cell lines secreting McAb against FB 1 . The method of multiple subclones was used to select cell lines stably secreting McAb. McAbs was got from ascites and purified. The subclass of antibody was measured and the molecular weight was identified. The specificity and sensitivity of McAb were identified with indirect competitive inhibition ELISA. Results : The results of serum from immuned mice showed that after five times of immunization the titer stables at 1A—10 -6 , and the McAb belongs to IgG1 subclass, the light chain was Io, the molecular weight of heavy and light chain were 55kDa and 32kDa, respectively. ELISA results showed that McAb could react with FB 1 . The linear range indirect competitive inhibition ELISA is 10-500ng/ml. Conclusion : The monoclonal hybridoma cell lines and the high specificity,high sensitivity of FB1-McAb was got.