Effect of temperature and salts on niosome-bound anti-cancer drug along with disruptive influence of cyclodextrins

Abstract Encapsulation of a persuasive anticancer drug (Sanguinarine, SGR) within microheterogeneous environment of niosome has been investigated. Utilizing steady-state and time-resolved spectroscopic methods the effects of extrinsically added salts and temperature on the photophysical properties of niosome-bound bio-active drug have been explored thoroughly. The prototropic (alkanolamine⇌ iminium) equilibrium of SGR is found to be preferentially favored toward the neutral form inside the hydrophobic interior of niosome. With addition of salts and increment of temperature the reverse tendency of stabilization of the cationic species is observed which can be explained on the basis of degree of water penetration of water molecules to the hydration layer of niosome. Furthermore, drug sequestration has been investigated via disruption of niosome applying cyclodextrins (CDs). Exploration of the effect of CDs (β-CD and γ-CD) on the niosome aids to have knowledge of the effect of CDs on cell membrane. In addition, the differential rotational relaxation behavior of SGR at various environmental circumstances has been observed to substantiate with other experimental results.