Expression and characterization of the key enzymes involved in 2-benzoxazolinone degradation by Pigmentiphaga sp. DL-8
2018
Abstract In this study, the key enzymes involved in 2-benzoxazolinone (BOA) degradation by Pigmentiphaga sp. DL-8 were further verified and characterized in Escherichia coli . By codon optimization and co-expression of molecular chaperones in a combined strategy, recombinant BOA amidohydrolase (rCbaA) and 2-aminophenol (2-AP) 1,2-dioxygenase (rCnbC α C β ) were expressed and purified with the highest activity of 1934.6 U·mg protein −1 and 32.80 U·mg protein −1 , respectively. BOA could be hydrolyzed to 2AP by rCbaA, which was further transformed to picolinic acid by rCnbC α C β based on identified catalytic product. The optimal pH and temperature for rCbaA are 9.0 and 55 °C with excellent stability for catalytic environments, and the residual activity was >50% after incubation at temperatures α C β composed of α-subunit (33 kDa) and β-subunit (38 kDa) showed poor stability against environmental factors, including temperature, pH, metal ions and chemicals.
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