Development and validation for the determination of total flavonoids and luteolin from Pteris multifida Poir
2017
Objective
To determine the content of total flavonoids and luteolin from Pteris multifida Poir.
Methods
The content of total flavonoids was determined by gradient elution of macroporous resin D101 and ultraviolet spectrophotometry. The content of luteolin was determined by HPLC. The analysis was performed on a RP-C18 column(4.6 mm×250 mm, 5 μm) with aceconitrile-0.2% phosphoric acid (35:65) as the mobile phase at a flow rate of 1.0 ml/min, and 30 ℃ temperature.
Results
The detection of wave length was set at 349 nm. The content of luteolin was 0.015%, 0.019%, 0.016%, and the content of total flavonoids was 0.015%, 0.019%, 0.016%, respectively.
Conclusions
The method is suitable for the determination of flavonoids componets from Pteris multifida Poir.
Key words:
Pteris multifida; Flavones; Chromatography, high pressure liquid; Luteolin
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