Axial resolution enhancement for planar Airy beam light-sheetmicroscopy via the complementary beam subtraction method
2021
Airy beam light-sheet illumination can extend the field of view
(FOV) of light-sheet fluorescence microscopy due to the unique propagation
properties of non-diffraction and self-acceleration. However, the side
lobes create undesirable out-of-focus background, leading to poor axial
resolution and low image contrast. Here, we propose an Airy complementary
beam subtraction (ACBS) method to improve the axial resolution while
keeping the extended FOV. By scanning the optimized designed complementary
beam that has two main lobes (TML), the generated complementary
light-sheet has almost identical intensity distribution to that of the
planar Airy light-sheet except for the central lobe. Subtraction of the
two images acquired by double exposure respectively using the planar Airy
light-sheet and the planar TML light-sheet can effectively suppress the
influence of the out-of-focus background. The axial resolution improves
from ∼4µm to 1.2 µm. The imaging performance was demonstrated by
imaging specimens of aspergillus conidiophores and GFP labeled mouse brain
section. The results show that the ACBS method enables the Airy beam
light-sheet fluorescence microscopy to obtain better imaging
quality.
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