Methods to Study the PVY Population in the Potato

The PVY population in the potato has been studied continuously using tobacco bait plants in potato fields at Mlochow since 1980 at two-year intervals and in potato tuber samples collected from different regions of Poland since 2001 yearly. The paper presents the combined biological, serological and molecular assays for PVY identification and strain classification. Biologically, PVY strains are defined with respect to their ability to elicit hypersensitive resistance (HR) mediated by N genes in differential potato cultivars (King Edward, Desiree and Pentland Ivory) and to symptoms in the tobacco (cultivar Samsun). Serologically, an ELISA assay based on polyclonal or monoclonal cocktail antibodies recognizes all PVY strain types, while the specific monoclonal antibodies help to recognize PVYN or PVYᴼ/PVYCstrains. Multiplex RT-PCR, Real-time RTqPCR and sequencing-based assays are used to define the PVY genome structure. In the Polish population of PVY, the strains PVYᴼ, PVYᴺᵀᴺ, PVYᴺ⁻ᵂⁱ, PVYZ-NTN and PVYᴱwere identified, while the PVYCstrain was not detected.