Multifunctional Supplement is that the Key to Modulate Redox Signaling in Metabolic Syndrome Volunteers: Cellular Metabolic Index

Scientists around the world concur that maintaining proper levels of redox signaling, which often will mistakenly interpret as oxidative and nitrosative stress. So, identifying the roots of such condition allowed us to design a strategy of nutritional intervention. Utilizing this approach, we performed a randomized, controlled, long-term study of the effect of a multifunctional nutritional supplement on redox signaling and health markers in 48 healthy and pre-metabolic syndrome volunteers. The measurement of extended cellular metabolic index based on cellular and mitochondrial dependent ROS formation and respiratory activity was performed using electron spin resonance spectrometer NOXYSCAN. Spin probe 1-hydroxy-3-methoxycarbonyl-2.2.5.5-tetramethylpyrrolidine (CMH) and oxygen label NOX-14.1 were used. Health parameters (blood pressure, heart rate, lipid and glucose profile, bioavailability of circulating NO was analyzed at control day, after 6 and 12 weeks of twice daily supplementation. Long-term supplementation resulted in significant inhibition of metabolic activity (20.7% cellular and 42.6% mitochondrial). We also observed significant inhibition of extracellular NADPH oxidase dependent generation of superoxide, nearly complete inhibition of extracellular H2O2 formation as well as a 2.5 times increase in bioavailable NO. Evaluated health parameters were improved on healthy people with even greater effects on those with metabolic syndrome: significant improvements in lipid profiles (TC, LDL, HDL, VLDL and TG), glucose metabolism (glucose, insulin, HbA1c, HOMA, postprandial hyperglycemia), inflammatory resistance as well as expression of redox sensitive proteins as NRF2, eNOS, SOD1, HO1 were shown. For the first time, we demonstrate the effects of a multifunctional nutritional supplement containing minerals, vitamins, antioxidant and anti-inflammatory phytonutrients on cellular redox signaling in pre-metabolic syndrome volunteers. We observed strong correlation of extended cellular metabolic index with the more than 25 markers and the indicators of “optimal health” as lipid-, glucose-, inflammatory profile, expression of redox sensitive proteins and other functional cardiovascular parameters.