Production and identification of monoclonal antibody against N terminal of AIB1

Objective To make and identify the monoclonal antibody against AIB1-N. Methods BALB/c mice were immunized with purified GST-AIB1-N protein,McAb against AIB1-N was produced by hybridoma technique. ELISA and Western-blot were used to identify the immunoglobin subtype and specificity. Results A hybridoma cell was successfully produced to secrete the McAb against AIB1-N, which was identified to belong in IgG1 subtype. By western-blot, the McAb against AIB1 displayed strongly specificity and high affinity. Conclusion The McAb against AIB1 protein may be a useful tool for studying the biological properties of AIB1 expression and the clinical laboratory detection.