Polysaccharides from marine red algae Amphiroa rigida and their biomedical potential: An in-vitro study

Abstract Herein, for the first time, the novel polysaccharides isolated from marine red algae Amphiroa rigida (ARPS) and enhanced its antioxidant, antibacterial, cytotoxicity, and apoptotic potential. An ultra-sonication disruption (USD) technology was employed to extract the ARPS and the extraction conditions were optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The maximum ARPS yield (28.6%) was achieved with the solid-liquid ratio (1:70 mL), ultra-sonication power (65%), and extraction time (60 min), respectively. Fourier-transform infrared spectroscopy (FT-IR) spectrum reports indicated that the presence of sulfate residues. Furthermore, ARPS was exhibited great potential in-vitro antioxidant activity in DPPH (58.24 ± 0.65%) and ABTS+ scavenging (52.23±0.88%) activity and also have good antibacterial activity against Salmonella Typhi (22±0.4 mm). In addition, ARPS can inhibit human breast cancer cells (MCF-7) and the apoptosis effect was observed through AO-EB and Hoechst staining in a dose-depended on manner. These experimental reports indicate that the ARPS have great potential as pharmaceutical applications.