Microbacterium enclense sp. nov., isolated from sediment sample.

A novel bacterium (strain NIO-1002T) belonging to the genus Microbacterium was isolated from a marine sediment sample in Chorao Island, Goa Province, India. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Cells of this strain were Gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. It grew in 0–12 % (w/v) NaCl and at 25–37 °C, with optimal growth at 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1002T is associated with members of the genus Microbacterium, with highest sequence similarity with Microbacterium hominis CIP 105731T (98.1 %) and Microbacterium testaceum KCTC 9103T (98.0 %). Within the phylogenetic tree, this novel strain shared a branching point with M. hominis CIP 105731T. The DNA G+C content was 66.5 mol% and DNA–DNA hybridization relatedness between NIO-1002T, M. hominis CIP 105731T and M. testaceum KCTC 9103T was 39.0 ± 2.0 % and 41.0 ± 2.0 %, respectively. The major fatty acids were ai-C15 : 0, i-C16 : 0 and ai-C17 : 0 and the diamino acid in the cell-wall peptidoglycan of NIO-1002T was lysine. Data obtained from DNA–DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain NIO-1002T represents a novel species within the genus Microbacterium. The name Microbacterium enclense sp. nov. is proposed, with NIO-1002T ( = NCIM 5454T = DSM 25125T = CCTCC AB 2011120T) as the type strain.