Identification of 14−3−3ζ by Chemical Affinity with Salicylanilide Inhibitors of Interleukin-12p40 Production

Salicylanilides were found as selective inhibitors of interleukin-12p40 production in stimulated dendritic cells. The conversion of one of these bioactive salicylanilides into a comparably bioactive, chemically labeled derivative was achieved using a facile and systematic functional group derivatization strategy. This resulted in a tool reagent that was then employed in an affinity chromatography approach that resulted in the identification of the protein 14-3-3ζ as having selective affinity for the chromatography matrix that was derivatized with a salicylanilide that inhibited IL-12p40 production.