Multiparametric phenotyping and cell health analysis of immune cell sub-populations on a novel microcapillary cytometer (TECH1P.870)

Cellular health enumeration and assessment of heterogeneous cellular sample such as whole blood and PBMCs often require many colors to accurately characterize subpopulations and are critical in defining immune cell handling as well as in functional studies ranging from cytokine secretion to proliferation. Traditional multi-color flow cytometers have posed challenges in multi-parametric experiments, managing compensation, and analyzing results. Existing solutions are expensive, cumbersome to run and require extensive training or dedicated personnel. In this study, we will present data from simplified methods allowing identification of multiple subsets including T,B, NK, naive and memory, monocytes, and granulocytes while simultaneously assessing cell health for studies with activation agents, cytotoxic treatments or culture conditions on a novel instrument platform, the guava easyCyte™ 12. The guava easyCyte™ 12 is a novel small footprint cytometer, with 3 lasers including blue, red and violet. This allows for data on up to 12 parameters in a microplate or tube-based format. The system is based on principles of microcapillary cytometery, provides absolute count and percentage data without external beads, uses low sample volume and uses flexible and intuitive software. The combination of multicolor antibody panel and cell health markers with the powerful instrument platform enables obtaining critical sub-population level information for PBMC and whole blood samples.