Genetic diversity in maize dent landraces assessed by morphological and molecular markers
2013
Maize Research Institute “Zemun Polje” genebank maintains a collection of
landraces grouped into 18 agro-ecological collected from ex-Yugoslavia
territories. The application and comparison of different marker systems are
important for the characterization and use of maize landraces in breeding
program, as potential sources of desirable traits. In this study, 15
morphological traits, 7 RAPD primers and 10 SSR primer pairs were applied to
i) to determine genetic distance between 21 maize dent landraces and ii)
compare results obtained on morphological and molecular markers. Phenotypic
analysis showed high level of heterogeneity between landraces. Higher level
of genetic diversity was obtained with SSR than with RAPD. Genetic distance
mean value for RAPD data was 0.35 i.e. for SSR 0.48. Based on the
morphological traits and molecular markers, unweighted pairgroup method
(UPGMA) analysis was applied for cluster analysis, using statistical NTSYSpc
program package. Cluster analysis of morphological and molecular markers
distances did not show the same population grouping. Better agreement with
agro-ecological data was obtained with RAPD markers. Correlations between
dissimilarity matrices for different types of markers were low. Data obtained
in this work could be useful for further study of a larger number of
landraces, and conservation of genetic resources and their genetic diversity.
[Projekat Ministarstva nauke Republike Srbije, br. TR31028: Exploitation of
maize diversity to improve grain quality and drought tolerance]
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
23
References
7
Citations
NaN
KQI